Journal: BMC biology

Article Title: Single-cell expression profile of Drosophila ovarian follicle stem cells illuminates spatial differentiation in the germarium.

doi: 10.1186/s12915-023-01636-9

Figure Lengend Snippet: Fig. 8 Expression patterns of selected genes. A–F Expression patterns of named gene products or derivatives in germaria co-stained with DAPI (blue in A–D) to reveal all nuclei and antibody to Fas3 (red in A–D; white in E–G) to allow location of the anterior edge of strong Fas3 expression (yellow arrows). A–A” Semaphorin 1a-GFP protein fusion (green) was detected on the surface of somatic cells; levels were low in region 1 (extreme anterior, left), peaked in region 2a ECs, FSCs, and early FCs, and declined in region 3 (posterior of the germarium) and the first budded egg chamber. B–B” Dystroglycan, detected by an antibody to the C-terminus (green), which connects the plasma membrane to ECM components, was seen lining the germarial wall from region 2a (white arrowheads in B’) and continuing posterior, consistent with its expected role and expression in all FSCs, early FCs, and a limited number of the most posterior ECs. Expression was also seen in the muscle sheath (cyan arrowheads in B). C–C” A Plum-GFP protein fusion (green) encoded by a large BAC genomic transgene was expressed most prominently in somatic cells, with highest levels in the FSC region and further posterior. D–D” A Cyclin B-GFP protein fusion showed occasional expression in layer1 FSCs (white arrow in D’ and D”) immediately anterior to the Fas3 border and was seen at similar levels in most FCs. Absence in some FCs is most likely due to protein degradation in G1 and S phases. Expression at the anterior of the germarium is within germline cells. E–G Santa Maria-Gal4 > UAS-RFP produced no RFP signal in most germaria. In other germaria, few cells expressed detectable RFP, including germaria with RFP signal in E a layer 1 FSC (white arrow) and a neighboring FC, F, G an FC adjacent to the Fas3 border, and G another early FC anterior to region 3. All bars, 20 µm

Article Snippet: Each ovary pair was then broken apart in PBS on a slide and mounted using DAPI fluoromountG mounting medium (Southern BioTech, OB010020).

Techniques: Expressing, Staining, Clinical Proteomics, Membrane, Produced

Journal: Biochemical and biophysical research communications

Article Title: Intravitreal indocyanine green is toxic to the retinal cells.

doi: 10.1016/j.bbrc.2024.150872

Figure Lengend Snippet: Fig. 3. Cell apoptosis assay by TUNEL in rat retinas 30 min after intravitreal injection of ICG solvents (GS) or ICG solutions. The rats were injected intravitreally with different concentrations of ICG + GS and GS for 30 min. The apoptotic cells (green) was detected with TUNEL, and the nucleus (blue) was stained with 4′,6-dia midino-2-phenylindole (DAPI). NC: negative control, PC: positive control, N: normal control, NG: 5 mM GS, GS-1: 92.5 mM GS, ICG-1: 0.43 mM ICG + 92.5 mM GS, GS-2: 185.02 mM GS, ICG-2: 0.86 mM ICG + 185.02 mM GS. GS: glucose solution, ICG: indocyanine green. Scale bar: 100 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Cell Counting Kit-8 (CCK-8) (Cat# 40203), antifade mounting medium DAPI Fluoromount-G (Cat# 36308ES11) and OCT compound (Cat# 36309ES61) were purchased from Yeasen Biotechnology (Shanghai) Co., Ltd. (Shanghai, China).

Techniques: Apoptosis Assay, TUNEL Assay, Injection, Staining, Negative Control, Positive Control, Control

Journal: Biochemical and biophysical research communications

Article Title: Intravitreal indocyanine green is toxic to the retinal cells.

doi: 10.1016/j.bbrc.2024.150872

Figure Lengend Snippet: Fig. 5. Cell apoptosis assay by TUNEL in rat retinas 60 min after intravitreal injection of ICG solvents (GS) or ICG solutions. The rats were injected intravitreally with different concentrations of ICG + GS and GS for 60 min. The apoptotic cells (green) were detected with TUNEL, and the nucleus (blue) was stained with 4′,6- diamidino-2-phenylindole (DAPI). NC: negative control, PC: positive control, N: normal control, NG: 5 mM GS, GS-1: 92.5 mM GS, ICG-1: 0.43 mM ICG + 92.5 mM GS, GS-2: 185.02 mM GS, ICG-2: 0.86 mM ICG + 185.02 mM GS. GS: glucose solution, ICG: indocyanine green. Scale bar: 100 μm. (For interpretation of the ref erences to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Cell Counting Kit-8 (CCK-8) (Cat# 40203), antifade mounting medium DAPI Fluoromount-G (Cat# 36308ES11) and OCT compound (Cat# 36309ES61) were purchased from Yeasen Biotechnology (Shanghai) Co., Ltd. (Shanghai, China).

Techniques: Apoptosis Assay, TUNEL Assay, Injection, Staining, Negative Control, Positive Control, Control